An Improvised Medium for Axenic Cultivation of Acanthamoeba spp.
نویسندگان
چکیده
منابع مشابه
enrichment of acanthamoeba culture medium using tyis 33 medium: a step toward a successful axenic cultivation
background: acanthamoeba-related disease have a poor prognosis according to many previous studies. thus researches regarding biochemical and molecular aspects of this organism are a high priority. to this end achieving high amount of amoebae in culture is the first step for such studies. the main aim of the present research was to address the usage of tyi-s-33 (tripticase, yeast extract, iron-s...
متن کاملAxenic Cultivation and Pathogenic Assays of Acanthamoeba Strains Using Physical Parameters
BACKGROUND The main goal of the present study was to set up an axenic cultivation of Acanthamoeba and assess the pathogenic ability of T4 genotypes from different clinical and environmental strains of Acanthamoeba using two physical assays. METHODS Sixteen Acanthamoeba isolates including 10 environmental and 6 clinical strains were cultured axenically. Axenic cultivation was performed using P...
متن کاملInfection in a rat model reactivates attenuated virulence after long-term axenic culture of Acanthamoeba spp
Prolonged culturing of many microorganisms leads to the loss of virulence and a reduction of their infective capacity. However, little is known about the changes in the pathogenic strains of Acanthamoeba after long culture periods. Our study evaluated the effect of prolonged culturing on the invasiveness of different isolates of Acanthamoeba in an in vivo rat model. ATCC strains of Acanthamoeba...
متن کاملContinuous axenic cultivation of Pneumocystis carinii.
Continuous axenic culture of Pneumocystis carinii has been achieved. A culture vessel is used that allows for frequent medium exchange without disturbance of organisms that grow attached to a collagen-coated porous membrane. The growth medium is based on Minimal Essential Medium with Earle's salt supplemented with S-adenosyl-L-methionine, putrescine, ferric pyrophosphate, N-acetyl glucosamine, ...
متن کاملaxenic cultivation and pathogenic assays of acanthamoeba strains using physical parameters.
background: the main goal of the present study was to set up an axenic cultivation of acanthamoeba and assess the pathogenic ability of t4 genotypes from different clinical and environmental strains of acanthamoeba using two physical assays. methods: sixteen acanthamoeba isolates including 10 environmental and 6 clinical strains were cul-tured axenically. axenic cultivation was performed using ...
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ژورنال
عنوان ژورنال: Indian Journal of Medical Microbiology
سال: 2017
ISSN: 0255-0857
DOI: 10.4103/ijmm.ijmm_17_151